How can we produce monoclonal antibody using e.coli cell? what is the prime application of that?

how far these monoclonal antibody useful and can you please explain any one aplication surrounded by detail.How can we produce monoclonal antibody using e.coli cell? what is the prime application of that?
this question can not be answered so glibly, because of the large concepts and applications of the monoclonal antibody. although, I will try to tender you the main Idea & push you to swot more.
mono clonal antibodies
* definition: they are antibodies that work against one antigen, and produced outside the B cell and outside the body of animal artificially.
* way of production: by taking the gene of this antibody from the innovative producing cell, then bond it with the DNA of E.coli later instesd of secreting the unproved substace (what ever it was), the bacteria will bury the antibody according to the information on the trasnplanted gene.
* application: one of these application is the tests that used to detect different proteins contained by the laboratory (e.g. looking for the antigen of hepatitis in blood specimen)
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